Regulatory

Part:BBa_K3621011

Designed by: Sangita Vasikaran   Group: iGEM20_MIT   (2020-10-04)


CasEcutsiteOFFCas6

With cleavage of this cutsite upstream of endoribonuclease Cas6, we can create an "OFF" switch for Cas6 translation. Inspired by 10.1101/2019.12.15.867150


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 525
    Illegal PstI site found at 549
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 525
    Illegal PstI site found at 549
    Illegal NotI site found at 106
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 525
    Illegal PstI site found at 549
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 525
    Illegal PstI site found at 549
    Illegal NgoMIV site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None