Reporter

Part:BBa_K362063

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)

yaiA promoter - RBS - GFP - Term - Term

This composite part contains yaiA promoter(BBa_K362006), RBS(BBa_K121013), GFP(BBa_K121013) and two terminators on low copy vector(BBa_K121013).

yaiA shows OxyR-dependent induction of expression by hydrogen peroxide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 351
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 668
    Illegal BamHI site found at 141
    Illegal BamHI site found at 657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 351
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1360


[edit]
Categories
Parameters
None