Composite

Part:BBa_K3619030

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-10-15)


Enterokinase Fusion Protein

Encodes Enterokinase fused to Thioredoxin 1 with a cleavage site in between. Enterokinase will be able the cleave the site itself and will then be a fully active Enterokinase. Expression of fully active enterokinase have been previously expressed in E. coli BL21 [1][2].

Enterokinase is a protease that will specifically cleave a site of Asp-Asp-Asp-Asp-Lys.

Sources:

1) https://www.sciencedirect.com/science/article/pii/S1046592802000128#FIG2

2) https://www.jbc.org/content/268/31/23311.long

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553


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