Part:BBa_K3619009

Gene AFP1 encoding the antimicrobial peptide Rs-AFP1.

The AFP1 gene encoding the Rs-AFP1 poly peptide which has a shown antimicrobial and fungicidal activity. It has the highest activity towards filamentous fungi. For tested microbes the half maximal inhibitory concentration (IC50) was ranging from 2-100ug/ml. However, when adding salt the IC50 was significantly increased [1].

The peptide has 4 disulfide bridges which may be problematic in bacterial expression.

For more information of the peptide, see Uniprot record P 69241 [2].

Sources:

1.https://www.researchgate.net/publication/21515511_Analysis_of_two_novel_classes_of_plant_antifungal_proteins_from_Radish_Raphanus_sativus_L_seeds

2.https://www.uniprot.org/uniprot/P69241

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]