Part:BBa_K3619006

cDNA encoding the ani microbial peptide EAFP1.

cDNA encoding the protein Antifungal peptide 1 (EAFP1). The protein binds chitin and has a broad antifungal toxicity, it has proven antifungal activity against several plant pathogens [1].

The half maximal inhibitory concentration (IC50) against testes species was between 35 to 177 ug/ml. The proteins antifungal activity is sensitive to Ca ions, which could 100-fold the IC50 at a concentration of 5mM calcium. EAFP1 has no shown inhibitory activity towards bacteria. [2].

The protein contains 5 disulfide bridges, which may be problematic in bacterial expression.

Sources:

1. uniprot.org/uniprot/P83596

2. https://pubmed.ncbi.nlm.nih.gov/12067732/

For more information about the protein , visist the uniprot record P83596 [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]