Part:BBa_K3619003

cDNA encoding the protein enterokinase.

cDNA encoding the catalytic subunit of bovine enterokinase, a protease responsible for activation of trypsinogen by a highly specific cleavage of the aa-sequence Asp-Asp-Asp-Asp-Lys. The high specificity makes it an appropriate protease for cleaving fusion proteins [1].

The enterokinase light chain have been previously expressed successfully in E. coli BL21 (DE3) and was also successful in cleaving a fusion protein. Is was expressed as a fusion protein with thioredoxin A as the partner (see part BBa_K..1) [2].

For more information of the protein see Uniport record E6Y432 [3].

Sources:

1. https://doi.org/10.1016/S1046-5928(02)00012-8

2. https://www.jbc.org/content/268/31/23311.long

3. https://www.uniprot.org/uniprot/E6Y432

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 211