Part:BBa_K3619002

cDNA encoding the protein glutathione S-transferase (GST)

The gst gene, a protein coding sequence coding for the protein glutathione-S-transferase (GST). GST enzymes are previously known to be important for detoxication of electrophiles [1].

GST can be used in a fusion protein for higher level of protein expression and easier purification of the target protein. The fusion protein can be purified by affinity chromatography using the enzymatic activity of GST [2]

GST have been used as a fusion protein for better expression of small peptides. It can aid in folding of the peptide and increase the solubility of the expressed product. [3]

For more information of the protein, visit the Uniprot record Q1RBG0 [4]

Sources:

1. https://doi.org/10.1016/S0027-5107(01)00206-8

2. https://doi.org/10.1002/0471140864.ps0606s52

3. https://doi.org/10.1016/j.pep.2017.08.003

4. https://www.uniprot.org/uniprot/Q1RBG0

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 156
  
• 1000
  COMPATIBLE WITH RFC[1000]