Tag

Part:BBa_K3612015

Designed by: Jesus Durand, Matias Rojas, Diego Benites, Maria Castromonte   Group: iGEM20_UPCH_Peru   (2020-10-23)


Signal Peptide (PhDsbA)

PhDsbA is a signal peptide for periplasmic secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The N-terminal leader peptide, called, PhDsbA is used for periplasmic secretion (1, 2). It was reported that PhDsbA allow the successful translocation of a heavy-chain antibody fragment into the periplasmic space of Pseudoalteromonas haloplanktis TAC125 (1, 2). Our team will use this part for periplasmic secretion of AFPs when expressing in Pseudoalteromonas nigrifaciens.

References

(1) Giuliani M, Parrilli E, Sannino F, Apuzzo G, Marino G, Tutino ML. Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125. Methods Mol Biol. 2015;1258:243–57.

(2) Madonna S, Papa R, Birolo L, Autore F, Doti N, Marino G, et al. The thiol-disulfide oxidoreductase system in the cold-adapted bacterium Pseudoalteromonas haloplanktis TAC 125: Discovery of a novel disulfide oxidoreductase enzyme. Extremophiles. 2006 Feb 23;10(1):41–51.


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