DNA

Part:BBa_K3602013

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs16901979-C Target Cas12a

The target DNA sequence includes the SNP rs6983267 with some surrounding nucleotides as if it was real DNA. The sequence that is targeted with the sgRNA has the PAM sequence TTTA and is 24 bp long. This is the non-risk allele with a C instead of A and is used as a control. The risk allele sequence is almost similar to this and is called (BBa_K3602012).

The sgRNA for this sequence was made by IDT just by uploading the sequence with a PAM and then specify the applied Cas-protein.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 44
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None