RNA

Part:BBa_K3602006

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs6983267-T Target Cas13a

This part includes the reverse complementary RNA sequence for the SNP rs6983267. This SNP is associated with increased risk for prostate cancer and can therefore be used for risk assessment tests for prostate cancer. This sequence is the non-risk allele and can therefore be used as a control. This sequence binds complementary to the rs6983267-T SNP sgRNA (BBa_ K3602008) which can be used together with cas13a (BBa_K3602020).

The part has a T7 promoter, in the beginning, enabling it for in vitro transcription from DNA to mRNA.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None