RNA

Part:BBa_K3602005

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


ERG Target Cas13a

This part contains the reverse complementary RNA sequence for the ETS transcription factor ERG, a part of the TMPRSS2:ERG fusions transcript. It is coupled with a T7 promoter upstream of it. The part can therefore be in-vitro transcribed to an ERG mRNA similar to the one found in humans. This sequence binds complementary to the ERG sgRNA (BBa_ K3602002) which can be used together with cas13a (BBa_K3602020). The mRNA is only present in the urine of patients with malignant prostate cancer and it can therefore be used as a biomarker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None