RNA

Part:BBa_K3602004

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs6983267-G sgRNA Cas13a

This sgRNA was designed for the rs6983267 SNP to match the risk G allele. The mutation matches in the gRNA’s seed sequence of the spacer so a mismatch will be poorly tolerated.

The beginning of the sequence is the T7 promoter which enables it for in-vitro transcription. This is followed by a Cas13a scaffold sequence which fuses the sgRNA to the Cas13a protein. In the sgRNA, the spacer sequence is the reverse complement of the target thus activating the Cas13a upon binding of the target. The matching target sequence can be found as (BBa_K3602007) and is used as a control to test the activation of the Cas13a protein. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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