RNA

Part:BBa_K3602002

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


ERG sgRNA Cas13a

This part contains a sgRNA sequence compatible with the Cas13a protein (BBa_K3602001). The sgRNA sequence is targeting the ETS transcription factor ERG on mRNA level. This mRNA is only present in the urine of patients with malignant prostate cancer and it can therefore be used as a biomarker.

The beginning of the sequence is the T7 promoter which enables it for in vitro transcription. This is followed by a cas13a scaffold sequence which fuses the sgRNA to the Cas13a protein. In the sgRNA, the spacer sequence is the reverse complement of the target. The matching target sequence can be found as (BBa_K3602005) and is used as a control to test the activation of the Cas13a protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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