Reporter

Part:BBa_K360125

Designed by: Claudia Ivonne Hernandez Armenta, Jorge Zepeda   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-22)


trpL promoter + cI inverter + GFP

The expression of the trp operon is repressed 300-fold by TrpR. TrpR binds at the trpLp promoter, possibly directly blocking binding by RNA polymerase Klig LS,1988.

The blue light sensor LovTAP is a chiameric protein that contains the TrpR DNA-binding domain, so that is expected to regulate trpL promoter as the native TrpR transcriptional regulator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1715


[edit]
Categories
Parameters
None