Device

Part:BBa_K360122

Designed by: Claudia Ivonne Hernandez Armenta   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-21)

Promoter + RBS + Lumazine + terminator

Lumazine is a Protein that affect the wavelength of the emitted light from the LuxABCDE operon, it has been isolated from Photobacterium phosphoreum. The lumazine proteins shift the color of the light to shorter wavelengths than 490 nm, around 475 nm.

Lumazine is already at the registry page, submitted by Edinburgh team 2009. See the Part entry BBa_K216007.

Strong RBS (BBa_B0034) with a spacer of seven bases between the RBS and the start codon which makes more efficient the transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None