Primer

Part:BBa_K360114

Designed by: Mariana Ruiz Velasco Leyva   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-15)


Primer to change RBS of P. pyralis luciferase

As reported in the design notes of part BBa_K216015, its sequencing shows an unexpected insertion of 6 bases, ACCACC, after the scar between the RBS and the ATG of the luciferase coding sequence; that is, the sequence reads ACTAGACCACCATG rather than ACTAGATG. This makes the RBS 12 bases from the ATG, somewhat more than the optimum. This primer intends to reestablish the efficiency.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None