Regulatory
Part:BBa_K360023
Designed by: Claudia Ivonne Hernandez Armenta, Jorge Zepeda Group: iGEM10_UNAM-Genomics_Mexico (2010-10-21)
trpL promoter
The expression of the trp operon is repressed 300-fold by TrpR. TrpR binds at the trpLp promoter, possibly directly blocking binding by RNA polymerase Klig LS,1988.
The blue light sensor LovTAP is a chiameric protein that contains the TrpR DNA-binding domain, so that is expected to regulate trpL promoter as the native TrpR transcriptional regulator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 23
Illegal SpeI site found at 31 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 23
Illegal SpeI site found at 31 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 23
Illegal SpeI site found at 31 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 23
Illegal SpeI site found at 31 - 1000COMPATIBLE WITH RFC[1000]
References
Klig LS., Carey J., Yanofsky C., 1988, trp repressor interactions with the trp aroH and trpR operators. Comparison of repressor binding in vitro and repression in vivo., J Mol Biol. 202(4):769-77.
[http://regulondb.ccg.unam.mx/operon?term=ECK120014577&format=jsp#trpLp RegulonDB]
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Categories
Parameters
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