Part:BBa_K3596050
BIOT RBS mut3
BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.
Figure 1: A Construction of BIOT gene circuit
In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system. This part codes for BIOT with the ppdA RBS replaced by hofM RBS mut1.The e-PN productivity of this part is shown in figure 2.
Figure 2: Different pilin productivity of engineered BIOT system with different RBS.
References
[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2637
Illegal PstI site found at 8227 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 8227
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2227
Illegal BglII site found at 2691
Illegal BglII site found at 4492
Illegal BamHI site found at 907
Illegal BamHI site found at 3016 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2637
Illegal PstI site found at 8227 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2637
Illegal PstI site found at 8227
Illegal NgoMIV site found at 1134
Illegal NgoMIV site found at 5979
Illegal AgeI site found at 980
Illegal AgeI site found at 1273
Illegal AgeI site found at 1836
Illegal AgeI site found at 4460
Illegal AgeI site found at 6748 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 7754
Illegal SapI.rc site found at 4096
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