Coding

Part:BBa_K3596007

Designed by: Xinyu Liu   Group: iGEM20_GreatBay_SZ   (2020-10-21)

ppdA+ppdB+ygdB+ppdC+gspO

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.

This part codes for the assembly machinery of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires(e-PNs), a part of the BIOT system
Figure 1: A Construction of BIOT gene circuit

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 764
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1770


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Categories
Parameters
None