Composite

Part:BBa_K3594001

Designed by: Runhan Luo   Group: iGEM20_SHSBNU_China   (2020-10-23)


Heat Responsive Suicide Switch

This composite part is used as the heat-responsive suicide switch, in which contains an promotor and the self-lysis gene BBa_K3594006. We expect that when the temperature reaches 37℃ or higher, the ribosome starts to translate the mRNA and the self-lysis protein would be expressed, resulting in the suicide of the bacteria.

gene circuit of heat-responsive suicide switch
the growth of BBa K3594001 overtime

The abscissa represents time, and the ordinate represents the growth of bacteria. It can be seen from the image that the OD value of the bacteria which is putted in 42°C drops at higher temperatures, which proves that temperature control suicide is effective.

In the 20h, the OD value of the bacteria which is putted in 42°C did not drop significantly as we imagine. Because the temperature in shaking incubator is lower than normal temperature, which may influence the result. Therefore, in the future, we would adjust equipment and measure OD again.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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