Intermediate
Part:BBa_K359009
Designed by: Cveta Manassieva Group: iGEM10_Waterloo (2010-09-02)
AIP sensor and consequent RFP reporter; contains FepA permeability pore
This part includes the S.aureus Agr quorum sensing sensor which recognizes the oligopeptide. The FepA pore allows for the oligopeptide to diffuse through the outer-membrane. Consequent RFP signal is produced, with an Excitation peak: 584 nm and Emission peak: 607 nm
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2115
Illegal NheI site found at 2138 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 426
Illegal BamHI site found at 1310 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2407
Illegal AgeI site found at 2773
Illegal AgeI site found at 3400
Illegal AgeI site found at 3918
Illegal AgeI site found at 5199
Illegal AgeI site found at 5311 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |