Intermediate

Part:BBa_K359009

Designed by: Cveta Manassieva   Group: iGEM10_Waterloo   (2010-09-02)


AIP sensor and consequent RFP reporter; contains FepA permeability pore

This part includes the S.aureus Agr quorum sensing sensor which recognizes the oligopeptide. The FepA pore allows for the oligopeptide to diffuse through the outer-membrane. Consequent RFP signal is produced, with an Excitation peak: 584 nm and Emission peak: 607 nm


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2115
    Illegal NheI site found at 2138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BamHI site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2407
    Illegal AgeI site found at 2773
    Illegal AgeI site found at 3400
    Illegal AgeI site found at 3918
    Illegal AgeI site found at 5199
    Illegal AgeI site found at 5311
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None