Part:BBa_K3590037
Scaffoldin for lysis of cyanobacteria
ScafL was designed to display proteins that would work together to lyse overpopulated cyanobacteria in affected water basins.
At the 5' end of ScafL there is the Pveg promoter followed by RBS R2. The CDS encodes several functional modules - the SacB signal sequence for extracellular transport, the His-tag for Western blot detection, followed by microvirin which is able to bind to the cyanobacterial cell wall and thus keep the target cells close to our host organism and to lysozyme. The microvirin module is connected by a linker to cohesin from Clostridium thermocellum which will interact with its dockerin counterpart connected to our lysozyme and thus attach this enzyme to the protein scaffold. Another linker then connects the previous parts of the fusion protein to three LysM domains that anchor the whole system to peptidoglycans in the cell wall of B. subtilis. At the 3' end of the CDS, there is a STOP codon.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1476
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1476
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1476
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1476
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 223
Illegal SapI.rc site found at 1538
| None |