Coding

Part:BBa_K3590011

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-09-01)


His-tag_v3

3x His-tag is a sequence coding 18 histidines in a row. It is used for the detection and purification of tagged proteins during western blotting or affinity chromatography.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None