Composite

Part:BBa_K3583012

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Physcomitrella patens PPK for expression in Physcomitrella patens

This construct was designed for expression in the moss Physcomitrella patens. It starts with the highly effective I2-10 promoter, followed by a RBS, the enzyme and the nos terminator. For more information on the single parts, check the information given on the pages of each biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3024
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2038
    Illegal XbaI site found at 2116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3024
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3024
    Illegal BglII site found at 2764
    Illegal BglII site found at 3502
    Illegal BamHI site found at 353
    Illegal BamHI site found at 998
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3024
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2038
    Illegal XbaI site found at 2116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3024
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2038
    Illegal XbaI site found at 2116
    Illegal NgoMIV site found at 695
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3271
    Illegal SapI.rc site found at 1103
    Illegal SapI.rc site found at 1649
    Illegal SapI.rc site found at 1858
    Illegal SapI site found at 3203


[edit]
Categories
Parameters
None