Composite

Part:BBa_K3583008

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Escherichia coli PPK mutant E173K with vacuolar import peptide

This construct was designed for expression in the moss Physcomitrella patens. It starts with the highly effective I2-10 promoter, followed by a RBS, the vacuolar signal petide, the enzyme, the plant specific insert and the nos terminator. For more information on the single parts, check the information given on the pages of each biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 972
    Illegal BamHI site found at 353
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal AgeI site found at 2326
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None