Coding
PPK E173K

Part:BBa_K3583005

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Escherichia coli PPK with E173K mutation

This Biobrick is the polyphosphate kinase from E. coli with a sequence change from glutamic acid to lysine in position 173. This mutation leads to a higher activity of the enzyme and to longer polyphosphate chains. Besides the mutation, the part is similar to BBa_K1807009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None