Signalling
VIP

Part:BBa_K3583003

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Vacuolar signal peptide for vacuolar import in Physcomitrella patens

For targeting of proteins to the plant vacuole, this part needs to be fixed to the n-terminus of the protein you want to translocate. Additionally you will need the 'Plant Specific Insert' which can be found under the part name: BBa_K3583004. The PSI only works for the moss Physcomitrella patens and needs to be fixed at the c-terminus. Don't forget to move potential stop codons from your protein behind the PSI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None