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Part:BBa_K3581014

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-23)


TorA signal peptide

TorA signal peptide is used to target protein secretion by having fully or partially folded proteins secreted to the periplasm. This part is flanked by HindIII cut sites because it allows the signal peptide to be cleaved out so the secretion method can be changed from extracellular to inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 139


References: 1. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38

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