Part:BBa_K3581004

aeBlue blue chromoprotein

aeBlue is a blue chromoprotein obtained from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue color. The part is flanked by Acc65I and AscI cut sites, which allows it to bind to the N or C terminus of the protein depending on our experimental goal.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References:

1. Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., Dyrhage, K., Gynnå, A., Ivermark, K., Lövgren, J., Törnblom, V., Virtanen, A., Lundin, E. R., Wistrand-Yuen, E., & Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of biological engineering, 12, 8. https://doi.org/10.1186/s13036-018-0100-0

2. https://parts.igem.org/Part:BBa_K864401