Tag

Part:BBa_K3581003

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


HlyA signal peptide

HlyA signal peptide used to target protein secretion via Type 1 secretion pathway by attaching signal peptide to C terminus. This allows the protein to be secreted and transported from cytoplasm to extracellular space. This part is flanked by HindIII cut sites because it allows the signal peptide to be cleaved out so the secretion method can be changed from extracellular to inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References: 1. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38

[edit]
Categories
Parameters
None