Tag

Part:BBa_K3581002

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


6x His Tag

6x His tag which is inserted in the C terminus of protein to allow purification and identification. The His tag can be identified and purified using nickel (II) ions in affinity purification columns or anti His tag antibodies. This part has a TEV protease cut site in front of the His tag sequence which can be used to cleave out the His tag after the protein has been purified.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References:

1. England, N. (n.d.). TEV Protease | NEB. Retrieved October 13, 2020, from https://international.neb.com/products/p8112-tev-protease#Product%20Information

2. Wikipedia. (2020, October 14). TEV Protease. Wikipedia. Retrieved October 13, 2020, from https://en.wikipedia.org/wiki/TEV_protease

[edit]
Categories
Parameters
None