Part:BBa_K3581000

Hagfish intermediate filaments alpha subunit

Hagfish are known to secrete slime when under attack and the slime contains a strong biopolymer thread called intermediate filaments. The intermediate filaments are mainly made up of two subunits, alpha and gamma respectively and this part codes for the alpha subunit. The alpha subunit will form linkage with the gamma subunit to form the hetero-dimeric intermediate filament proteins. As we wanted to include chromoproteins in our circuit, this part also includes a Acc65I cut site in front of the coding region and a AscI cut site after the coding region for inserting the chromoproteins in the N or C terminus of the intermediate filaments for pre-dyeing purposes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References: 1. Jing, F., A. Guerette, P., Pavesi, A., Horbelt, N., Chwee Teck, L., J. Harrington, M., & Miserez, A. (2017). Artificial hagfish protein fibers with ultra-high and tunable stiffness, 9(35), 12908–12915. The Royal Society of Chemistry.