Generator

Part:BBa_K3580103

Designed by: Yuya Otsuki   Group: iGEM20_Waseda   (2020-10-26)


A series of enzymes of the mevalonate pathway

It is a part composed of 7 enzymes of mevalonate pathwaywhose expression is regulated by PlacUV5, and LacI to enable IPTG induction of expression of those enzymes.This part is a converted version of pBbA5c-MevT-MBI created and used in a paper reported by Alonso-Gutierrez, Jorge et al. in 2013 to make Biobrick RFC 1000 compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1532
    Illegal XbaI site found at 5511
    Illegal XbaI site found at 6620
    Illegal XbaI site found at 8076
    Illegal XbaI site found at 8969
    Illegal SpeI site found at 10120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1532
    Illegal SpeI site found at 10120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1532
    Illegal BglII site found at 1541
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1532
    Illegal XbaI site found at 5511
    Illegal XbaI site found at 6620
    Illegal XbaI site found at 8076
    Illegal XbaI site found at 8969
    Illegal SpeI site found at 10120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1532
    Illegal XbaI site found at 5511
    Illegal XbaI site found at 6620
    Illegal XbaI site found at 8076
    Illegal XbaI site found at 8969
    Illegal SpeI site found at 10120
    Illegal NgoMIV site found at 1418
    Illegal NgoMIV site found at 5656
    Illegal AgeI site found at 3374
    Illegal AgeI site found at 6852
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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