Plasmid

Part:BBa_K3579022

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF0_T7TE-LuxIA_GH

This plasmid is a level 0 plasmid of the EpiFlex toolkit (MoClo for s. epidermidis) containing the T7 terminator part. This part is designed with fusion sites G and H which aims to place it in position 7 in the TU assembled in the level 1 plasmid.

This plasmid has a pPBEF_bb0 backbone with Ampicillin resistance gene for E. Coli. The plasmid is a E. coli -> S. epidermidis shuttle vectors. This means that they contain origins of replication for both E. coli and S. epidermidis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1783
    Illegal SpeI site found at 792
    Illegal SpeI site found at 2591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6472
    Illegal SpeI site found at 792
    Illegal SpeI site found at 2591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 777
    Illegal XhoI site found at 770
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1783
    Illegal SpeI site found at 792
    Illegal SpeI site found at 2591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1783
    Illegal SpeI site found at 792
    Illegal SpeI site found at 2591
    Illegal NgoMIV site found at 6620
    Illegal AgeI site found at 1129
    Illegal AgeI site found at 6089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 124
    Illegal SapI site found at 3934


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