Plasmid

Part:BBa_K3579021

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF0_mCherry_CG

This plasmid is a level 0 plasmid of the EpiFlex toolkit (MoClo for s. epidermidis) containing the mCherry CDS part. This part is designed with fusion sites C and G which aims to place it in position 3 to 6 in the TU assembled in the level 1 plasmid.

This plasmid has a pPBEF_bb0 backbone with Ampicillin resistance gene for E. Coli. The plasmid is a E. coli -> S. epidermidis shuttle vectors. This means that they contain origins of replication for both E. coli and S. epidermidis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2453
    Illegal SpeI site found at 1462
    Illegal SpeI site found at 3261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7142
    Illegal SpeI site found at 1462
    Illegal SpeI site found at 3261
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
    Illegal BamHI site found at 1447
    Illegal XhoI site found at 1440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2453
    Illegal SpeI site found at 1462
    Illegal SpeI site found at 3261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2453
    Illegal SpeI site found at 1462
    Illegal SpeI site found at 3261
    Illegal NgoMIV site found at 7290
    Illegal AgeI site found at 647
    Illegal AgeI site found at 1799
    Illegal AgeI site found at 6759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 794
    Illegal SapI site found at 4604


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