Plasmid

Part:BBa_K3579020

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF0_Sod_BC

This plasmid is a level 0 plasmid of the EpiFlex toolkit (MoClo for s. epidermidis) containing the SodA RBS part. This part is designed with fusion sites B and C which aims to place it in position 2 in the TU assembled in the level 1 plasmid.

This plasmid has a pPBEF_bb0 backbone with Ampicillin resistance gene for E. Coli. The plasmid is a E. coli -> S. epidermidis shuttle vectors. This means that they contain origins of replication for both E. coli and S. epidermidis. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1706
    Illegal SpeI site found at 715
    Illegal SpeI site found at 2514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6395
    Illegal SpeI site found at 715
    Illegal SpeI site found at 2514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 700
    Illegal XhoI site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1706
    Illegal SpeI site found at 715
    Illegal SpeI site found at 2514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1706
    Illegal SpeI site found at 715
    Illegal SpeI site found at 2514
    Illegal NgoMIV site found at 6543
    Illegal AgeI site found at 1052
    Illegal AgeI site found at 6012
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 47
    Illegal SapI site found at 3857


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