Plasmid

Part:BBa_K3579019

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF0_SarA_AB

This plasmid is a level 0 plasmid of the EpiFlex toolkit (MoClo for s. epidermidis) containing the SarA promoter. This part is designed with fusion sites A and B which aims to place it in position 1 in the TU assembled in the level 1 plasmid.

This plasmid has a pPBEF_bb0 backbone with Ampicillin resistance gene for E. Coli. The plasmid is a E. coli -> S. epidermidis shuttle vectors. This means that they contain origins of replication for both E. coli and S. epidermidis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1724
    Illegal SpeI site found at 733
    Illegal SpeI site found at 2532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6413
    Illegal SpeI site found at 733
    Illegal SpeI site found at 2532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
    Illegal XhoI site found at 711
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1724
    Illegal SpeI site found at 733
    Illegal SpeI site found at 2532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1724
    Illegal SpeI site found at 733
    Illegal SpeI site found at 2532
    Illegal NgoMIV site found at 6561
    Illegal AgeI site found at 1070
    Illegal AgeI site found at 6030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 65
    Illegal SapI site found at 3875


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