Regulatory

Part:BBa_K3579014

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_PxylA


This part is an inducible promoter (Inducible by xylR). It's codon optimized for S. epidermidis. It is EpiFlex (MoClo kit for S. epidermidis) compatible : It has fusion site level 0 A and B allowing its insertion in the level 0 backbone. It has fusion site level 1 A and B allowing its insertion in position 1 in the TU assemble in the level 1 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 1416


[edit]
Categories
//promoter
Parameters
chassisS. epidermidis