Reporter
Part:BBa_K3579011
Designed by: Amandine Maire Group: iGEM20_Paris_Bettencourt (2020-10-27)
EpiFlex_EYFP
This part is the coding sequence of the EYFP protein. It's codon optimized for S. epidermidis. I It is EpiFlex (MoClo kit for S. epidermidis) compatible : It has fusion site level 0 A and B allowing its insertion in the level 0 backbone. It has fusion site level 1 C and G allowing its insertion in position 3 to 6 in the TU assembled in the level 1 plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 13
Illegal BsaI.rc site found at 777
[edit]
Categories
Parameters
//classic/reporter
biology | Discosoma sp |
chassis | S. epidermidis |
color | Yellow |
emission | 527 |
excitation | 513 |