Part:BBa_K3579006

EpiFlex_GFP

This part is the coding sequence of the GFP protein. It's codon optimized for S. epidermidis. It is EpiFlex (MoClo kit for S. epidermidis) compatible : It has fusion site level 0 A and B allowing its insertion in the level 0 backbone. It has fusion site level 1 C and G allowing its insertion in position 3 to 6 in the TU assembled in the level 1 plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 444
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 13
  Illegal BsaI.rc site found at 743