Coding

Part:BBa_K3579000

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


mCherry

This part is the coding sequence of the mCherry protein. It's codon optimized for S. epidermidis. It's EpiFlex (MoClo kit for S. epidermidis) compatible : it aimed to be on position 3 to 6 of the TU assembled in the level 1 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 794


[edit]
Categories
//classic/reporter
Parameters
biologyDiscosoma sp
chassisS. epidermidis
colorRed
emission610
excitation587