Part:BBa_K3576000
OmpR-Strengthening biofilms system
It is the key part that is responsible for enhancing biofilm synthesis. The protein OmpR234 can activate the operon csgDEFG and the subsequent expression of downstream proteins increasing biofilm formation (Bamhart & Chapman 2006).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 211
- 1000COMPATIBLE WITH RFC[1000]
Results
1. Protein expression test
SDS-PAGE electrophoresis was used to check the expression of OmpR234 proteins. As shown in Figure 1, compared to the blank control, the lane contained OmpR234 protein (27 KDa) indicated that this protein has been successfully expressed.
2. Expression of OmpR234 Increases Biofilm Formation
To verify whether the insertion of the OmpR234 gene strengthens the production of biofilm, we mixed a commonly used dye, Congo Red (CR), with samples in a 2 mL-system, transferring them to 24-well microtiter plates and incubating with the glass cover for 3 days. If biofilms were present, they would absorb the dye and appear as red, so the strengthened biofilm production would show a higher concentration of dye. As shown in Figure 2, compared with the control groups, LB medium and E. Coli (BL21), the third group which overexpressed OmpR234 demonstrates a stronger red color, which indicated higher production of biofilm.
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