Composite

Part:BBa_K353004

Designed by: Alejandro Virrueta   Group: iGEM10_Stanford   (2010-10-25)


I0500 + sRNA2

This part is composed of an inducible arabinose promoter (I0500) and an sRNA sequence. This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). Arabinose induces the production of the sRNA2 transcript, and if no sRNA1 is present (see part ), this can then be translated into GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None