Composite

Part:BBa_K353003

Designed by: Alejandro Virrueta   Group: iGEM10_Stanford   (2010-10-25)


F2620 + RSID2 + B0034 + E1010 + B1006

This part is composed of an inducible AHL promoter (F2620), an RNA tag, a ribosome binding site (B0034), RFP (E1010), and a terminator (B1006). This is one of the four subsystems utilized in detecting a ratio (see Stanford iGEM 2010 project). AHL induces the production of an mRNA transcript, and if no sRNA2 is present (see part ), this can then be translated into RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 1754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
None