Composite

Part:BBa_K3524006

Designed by: Kong Yangyang   Group: iGEM20_Nanjing_high-school   (2020-10-18)


NahR-Psal-GADPH

The bio-sensor (nahR and Psal) for the detection of salicylic acid we adopted is originally on the chromosome of Pseudomonas Stutzeri (Bosch, Rafael, et al.). With the appearance of salicylic acid, the protein translated form nahR binds with Psal promoter and initiates the expression of the enzyme that can utilize the acid as one of the energy sources for Pseudomonas Stutzeri. In our design, we replaced the enzyme with an essential gene GADPH for the metabolism of Tatumella Citrea. Therefore, it can only live around the roots that produce salicylic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 1522
    Illegal AgeI site found at 1462
    Illegal AgeI site found at 1729
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None