Protein_Domain

Part:BBa_K3522000

Designed by: Kong Yangyang   Group: iGEM20_NOFLS_YZ   (2020-10-16)


FXR-LBD

Farnesoid X receptor (FXR) belongs to amember of nuclear receptor Superfamily and has been reported to be associated with glucose metabolism. For example, in FXR−/− mice, the level of hepatic gluconeogenic gene expression was decreased after fasting, while blood glucose level and the hepatic glucose production in response to pyruvate challenge were declined. BBa_K3522000 is FXR ligand binding domain(FXR-LBD), cloned into Nde1/XhoI sites of pET-15b vector to get the plasmid expressing N-terminal His-FXR-LBD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None