Coding

Part:BBa_K3514001

Designed by: Oona Jung   Group: iGEM20_BOKU-Vienna   (2020-10-22)


Protein Exo of the Lambda Red System, degrades dsDNA into ssDNA, leaves 3' ends

Lambda exonuclease 'Exo' binds non-sequence specific to dsDNA and degrades it to ssDNA, leaving 3' ends


Part of the Lambda Red recombination system, needed to degrade dsDNA aswell as loading beta onto the strand

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Exonuclease from bacteriophage lambda

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Categories
Parameters
None