Coding

Part:BBa_K3513005

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


β-Glucosidase with promoter

Constitutive promoter J23119 binds with the β-Glucosidase from Streptomyces coelicolor. In cellulose degradation, β-glucosidase is used to break cello-oligosaccharides into glucose. We use this part to accomplish the last step of cellulose degradation. The gene encoding β-glucosidase (BBa_K3279007) was obtained by PCR amplification from the genomic DNA of Streptomyces coelicolor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 1146
    Illegal AgeI site found at 227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 730
    Illegal BsaI.rc site found at 989


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Categories
Parameters
None