Coding

Part:BBa_K3513004

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


exoglucanase with signal peptide, linker and promoter

Constitutive promoter J23119 binds with the exoglucanase with signal peptide, which enables the engineered exoglucanase to be expressed in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 637
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 102
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 643
    Illegal NgoMIV site found at 1145
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690
    Illegal SapI.rc site found at 773


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Categories
Parameters
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