Coding

Part:BBa_K3513002

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


endoglucanase with signal peptide, linker and promoter

Constitutive promoter J23119 binds with our endoglucanase with signal peptide, which enables the engineered endoglucanase to be expressed in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 147
    Illegal NotI site found at 1291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1204
    Illegal BamHI site found at 340
    Illegal XhoI site found at 702
    Illegal XhoI site found at 951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 102
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 1407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 386


[edit]
Categories
Parameters
None